Stable transformation of trypanosomatids through targeted chromosomal integration of the selectable marker gene encoding blasticidin S deaminase
نویسندگان
چکیده
منابع مشابه
Over expression of the selectable marker blasticidin S deaminase gene is toxic to human keratinocytes and murine BALB/MK cells
BACKGROUND The blasticidin S resistance gene (bsr) is a selectable marker used for gene transfer experiments. The bsr gene encodes for blasticidin S (BS) deaminase, which has a specific activity upon BS. Therefore, its expression is supposed to be harmless in cells. The work reported on herein consisted of experiments to verify a possible toxicity of bsr on mammalian cells, which include severa...
متن کاملCharacterization of the Hansenula polymorpha PUR7 gene and its use as selectable marker for targeted chromosomal integration.
The Hansenula polymorpha genes encoding the putative functional homologs of the enzymes involved in the seventh and eighth step in purine biosynthesis, HpPUR7 and HpPUR8, were cloned and sequenced. An overexpression vector designated pHIPA4 was constructed, which contains the HpPUR7 gene as selectable marker and allows expression of genes of interest via the strong, inducible alcohol oxidase pr...
متن کاملNew positive/negative selectable markers for mammalian cells on the basis of Blasticidin deaminase-thymidine kinase fusions.
Two positive and negative selectable markers were created for use in mammalian cells. They are based on two genes for the resistance to Blasticidin S (BlaS) and on the thymidine kinase (Tk) gene of herpes simplex virus (HSV). The markers can be selected positively by their ability to induce BlaS resistance and negatively on the induced sensitivity towards gancyclovir (GANC). Both constructs are...
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چکیده ندارد.
15 صفحه اولChloroplast Transformation of Platymonas (Tetraselmis) subcordiformis with the bar Gene as Selectable Marker
The objective of this research was to establish a chloroplast transformation technique for Platymonas (Tetraselmis) subcordiformis. Employing the gfp gene as a reporter and the bar gene as a selectable marker, transformation vectors of P. subcordiformis chloroplast were constructed with endogenous fragments rrn16S-trnI (left) and trnA-rrn23S (right) as a recombination site of the chloroplast ge...
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ژورنال
عنوان ژورنال: FEMS Microbiology Letters
سال: 2000
ISSN: 0378-1097
DOI: 10.1016/s0378-1097(00)00159-2